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stratalinker uv crosslinker 2400  (Agilent technologies)


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    Structured Review

    Agilent technologies stratalinker uv crosslinker 2400
    Stratalinker Uv Crosslinker 2400, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stratalinker+2400+uv+crosslinker/pmc11316803-299-14-18
    Average 90 stars, based on 1 article reviews
    stratalinker uv crosslinker 2400 - by Bioz Stars, 2026-09
    90/100 stars

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    Irradiation:

    Article Title: Eukaryotic Elongation Factor 2 Kinase EFK-1/eEF2K promotes starvation resistance by preventing oxidative damage in C. elegans
    Article Snippet: .. For the somatic NER assay, L1 worms were synchronized overnight on unseeded NGM-l plates, then irradiated uncovered in a Stratalinker 2400 UV Crosslinker (Stratagene) with wavelength 254 nm light at specified dosages. ..

    Article Title: The unfolded protein response of the endoplasmic reticulum protects Caenorhabditis elegans against DNA damage caused by stalled replication forks
    Article Snippet: .. Uncovered NGM-lite plates were then placed in a Stratalinker 2400 UV Crosslinker (Stratagene) and irradiated with wavelength 254 nm light at 400 J/m 2 . ..

    Article Title: The chromodomain proteins, Cbx1 and Cbx2 have distinct roles in the regulation of heterochromatin and virulence in the fungal wheat pathogen, Zymoseptoria tritici
    Article Snippet: Cells were subjected to five-fold serial dilution and pinned onto the indicated YMS and PD agar (Formedium) plates with a 48-pin tool (Sigma). .. UV irradiation was achieved using a Stratalinker 2400 UV crosslinker (Stratagene). ..

    Concentration Assay:

    Article Title: NAT10-mediated ac 4 C modification promotes stemness and chemoresistance of colon cancer by stabilizing NANOGP8
    Article Snippet: Herein, we deployed TB Green Premix Ex Taq II (Takara Bio) for conducting RT-qPCR, setting 18S as an endogenous control. summarizes primer sequences. .. The experimental group-collected total RNA samples were diluted to an equal concentration and subjected to heat at 95 °C for 3 min, thereby uniformly distributing 2 μl of the diluted RNA onto a Hybond-N+ membrane (#RPN203B, GE Healthcare, Chicago, IL, USA) and cross-linking by employing a Stratalinker 2400 UV Crosslinker (1,200 μJ, 5 min; Agilent Technologies, Santa Clara, CA, USA). .. The membrane went through blockage utilizing 5 % bovine serum albumin (BSA) besides overnight incubation at 4 °C with an anti-ac 4 C antibody (ab252215, Abcam).

    Membrane:

    Article Title: NAT10-mediated ac 4 C modification promotes stemness and chemoresistance of colon cancer by stabilizing NANOGP8
    Article Snippet: Herein, we deployed TB Green Premix Ex Taq II (Takara Bio) for conducting RT-qPCR, setting 18S as an endogenous control. summarizes primer sequences. .. The experimental group-collected total RNA samples were diluted to an equal concentration and subjected to heat at 95 °C for 3 min, thereby uniformly distributing 2 μl of the diluted RNA onto a Hybond-N+ membrane (#RPN203B, GE Healthcare, Chicago, IL, USA) and cross-linking by employing a Stratalinker 2400 UV Crosslinker (1,200 μJ, 5 min; Agilent Technologies, Santa Clara, CA, USA). .. The membrane went through blockage utilizing 5 % bovine serum albumin (BSA) besides overnight incubation at 4 °C with an anti-ac 4 C antibody (ab252215, Abcam).

    Article Title: Cotton ( Gossypium hirsutum ) VIRMA as an N6-Methyladenosine RNA Methylation Regulator Participates in Controlling Chloroplast-Dependent and Independent Leaf Development.
    Article Snippet: Purified mRNA was first denatured by heating at 65 ◦C for 2 min, followed by chilling on ice directly. mRNA was spotted on a Hybond-N+ membrane (Amersham, USA) optimized for nucleic acid transfer. .. After UV crosslinking in a Stratalinker 2400 UV Crosslinker (Stratagene, La Jolla, CA, USA), the membrane was washed by PBST buffer (PBS with Tween-20), blocked with 5% of non-fat milk in PBST, and incubated with m6A-specific antibody (1:250; No. 202003, Synaptic Systems, Goettingen, Germany) overnight at 4 ◦C. ..

    Incubation:

    Article Title: Cotton ( Gossypium hirsutum ) VIRMA as an N6-Methyladenosine RNA Methylation Regulator Participates in Controlling Chloroplast-Dependent and Independent Leaf Development.
    Article Snippet: Purified mRNA was first denatured by heating at 65 ◦C for 2 min, followed by chilling on ice directly. mRNA was spotted on a Hybond-N+ membrane (Amersham, USA) optimized for nucleic acid transfer. .. After UV crosslinking in a Stratalinker 2400 UV Crosslinker (Stratagene, La Jolla, CA, USA), the membrane was washed by PBST buffer (PBS with Tween-20), blocked with 5% of non-fat milk in PBST, and incubated with m6A-specific antibody (1:250; No. 202003, Synaptic Systems, Goettingen, Germany) overnight at 4 ◦C. ..



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